cd68 (Bio-Rad)
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Structured Review
Bio-Rad
cd68
Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd68/product/Bio-Rad
Average 94 stars, based on 247 article reviews
Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd68/product/Bio-Rad
Average 94 stars, based on 247 article reviews
cd68 - by Bioz Stars,
2026-04
94/100 stars
Images
1) Product Images from "HDL Regulates TGFβ-Receptor Lipid Raft Partitioning, Restoring Contractile Features of Cholesterol-Loaded Vascular Smooth Muscle Cells"
Article Title: HDL Regulates TGFβ-Receptor Lipid Raft Partitioning, Restoring Contractile Features of Cholesterol-Loaded Vascular Smooth Muscle Cells
Journal: JACC: Basic to Translational Science
doi: 10.1016/j.jacbts.2025.101461
Figure Legend Snippet: Macrophage Markers Upregulated in Chol-Loaded hVSMCs Are Suppressed by HDL Through Restoration of TGFβ Signaling (A) hVSMCs were treated with Chol (5 μg/mL) or 0.2% bovine serum albumin (CT) for 48 hours. qPCR was performed to determine the expression of macrophage marker ( Cd68 ) and SMC marker ( Acta2 ). (B) hVSMCs were with treated as in A for 48 hours, then qPCR was performed to determine the expression of macrophage differentiation factor Klf4 . (C) hVSMCs were treated with Chol (5 μg/mL) for the indicated times, then KFL4 expression was determined by Western blotting. (D) Klf4 (60 nmol/L) or negative CT small, interfering RNA (siRNA) were transfected into hVSMCs for 48 hours. Then, transfected cells were treated as in B, followed by Western blotting for CD68 and KLF4. GAPDH was used as loading CT. (E) Chol-loaded cells (48 hours, 5 μg/mL) were incubated with Mir145 mimic (60 nmol/L) or CT mimic (60 nmol/LM) for 24 hours and the expressions of CD68, KLF4, and α-SMA determined with GAPDH as a loading CT. The P values for the comparisons between CT and Mir145 mimics are CD68 (0.025), KLF4 (0.018), and α-SMA (0.01). (F-I) hVSMCs were loaded with Chol (48 hours, 5 μg/mL) and were then either treated with HDL (50 μg/mL) for 24 hours or left untreated. Western blotting was performed to determine the expression of (F) KLF4 and (G) CD68. (H) hVSMCs were treated as in F and G, but in the presence or absence of TGFβR1i (50 ng/mL). Western blotting was performed to determine KLF4 expression. For data analysis, unpaired Student’s t -test was performed for comparing the means of 2 groups. For 2 or more independent groups, 2-way analysis of variance followed by Dunnett post hoc test was performed. Data are presented as the mean ± SEM of at least 3 independent experiments. P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001). Abbreviations as in , , and .
Techniques Used: Expressing, Marker, Western Blot, Small Interfering RNA, Transfection, Incubation
Figure Legend Snippet: HDL Increases the Expression of Acta2 Relative to That of CD68 in Atherosclerotic Mice (A) Schematic representation of experimental design. Note that ApoA1, which forms HDL particles in vivo, was injected after atherosclerosis progression (P) to induce regression (R). (B) Representative images from P and R mice that were sufficient ( Tgfβr2 +/+ ) or haplosufficient ( Tgfβr2 +/− ) for TGFβR2, showing the lineage-positive VSMCs (GFP + ) expressing macrophage marker or CD68 (red). Yellow color represents GFP-expressing CD68 + cells. (C) Quantification of GFP + /CD68 + . (D) Aortic digestion followed by cell sorting of GFP + cells was performed using flow cytometry to capture lineage-positive cells (GFP) expressing macrophage markers (CD11b and F4/80). (E) Total RNA was isolated from sorted cells and qPCR was performed to identify Acta2 expression. For data analysis of 2 or more independent groups, 2-way analysis of variance followed by Šídák multiple comparisons post hoc test was performed. Data are presented as the mean ± SEM (n = 5-6 mice per group). P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001). DAPI = 4ʹ,6-diamidino-2-phenylindole; other abbreviations as in , , and .
Techniques Used: Expressing, In Vivo, Injection, Marker, FACS, Flow Cytometry, Isolation
